Journal: Acta Pharmacologica Sinica

Article Title: Brain protection against ischemic stroke using choline as a new molecular bypass treatment

doi: 10.1038/aps.2015.104

Figure Lengend Snippet: Effects of oral choline treatment on angiogenesis in the ischemic brains. (A) Macroscopic images of the pial vasculature. (B) Microscopic images of ischemic cerebral cortex sections immunohistochemically stained using an anti-CD34 antibody to analyze vessel density, scale bar=20 μm. (C) Vessel density (n=6). (D) Number of microvessels in the cortex (n=3). Mean±SEM. bP<0.05, cP<0.01 vs sham; eP<0.05, fP<0.01 vs pMCAO.

Article Snippet: The capillary density was examined using a primary rabbit anti-rat CD34 antibody (Bioss, Beijing, China).

Techniques: Staining

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: In vivo analysis of vascularization and biocompatibility of electrospun polycaprolactone fibre mats in the rat femur chamber

doi: 10.1002/term.2868

Figure Lengend Snippet: HE staining, van Gieson staining, and immunohistochemical detection of CD 34: Representative histological stainings (a–o) 20 days after implantation into the femur chamber in Lewis rats. (a–c) HE staining of (1) granulation tissue, (2) implant, and (3) bone. (d–f) HE staining with higher magnification. (g–i) Collagen fibres were detected by van Gieson staining. (j–l) The presence of endothelial cells and accordingly the presence of vascular structures were confirmed by immunohistochemical detection of CD34. (m–o) Negative controls. Areas of the implant are marked (#), and arrows denote vascular structures [Colour figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: For detection of capillaries, endothelial cells were immunohistochemically stained using a rabbit anti‐rat CD34 antibody (Hoelzel Diagnostika Handels GmbH, Koeln, Germany).

Techniques: Staining, Immunohistochemical staining